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Anglický jazyk
Synthesis and enzymatic testing of reversible terminators for SBS
Autor: Angelika Keller
Based on the state-of-the-art DNA sequencing method - Sanger sequencing - the major aim of future research is to develop a cost-effective and gel electrophoresis-free sequencing method. These new sequencing technologies - such as the so-called sequencing-by-synthesis... Viac o knihe
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O knihe
Based on the state-of-the-art DNA sequencing method - Sanger sequencing - the major aim of future research is to develop a cost-effective and gel electrophoresis-free sequencing method. These new sequencing technologies - such as the so-called sequencing-by-synthesis method (SBS) - should be able to detect DNA mutations faster and more accurate than the currently available sequencing technique. The function of a reversible terminator used for SBS is as follows: 3'-modified, dye-labeled nucleotides are incorporated by the polymerase complementary to the DNA-template (as already known from Sanger sequencing) stopping the DNA-synthesis immediately. After detection of the fluorescent signal, the reversible terminator has to be cleavable in such a way (here: the 3'-modification) that the DNA-synthesis can continue (i. e. "reversible" termination). Thus SBS enables a step-by-step chain elongation with a direct read-out of the sequence via fluorescent signals. This PhD thesis covers the synthesis of 3'-modified nucleosides as key compounds for these terminators and the evaluation of suitable 3'-modification cleavage conditions.
- Vydavateľstvo: Südwestdeutscher Verlag für Hochschulschriften
- Rok vydania: 2010
- Formát: Paperback
- Rozmer: 220 x 150 mm
- Jazyk: Anglický jazyk
- ISBN: 9783838113555
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