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Anglický jazyk
Skeletal Muscle Regeneration in the Mouse
Autor: Michael Kyba
This volume focuses
on the cell biology and physiology of skeletal muscle regeneration. This Book
is a collection of classic and cutting edge protocols optimized for mice, but
in most cases adaptable to rat or...
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O knihe
This volume focuses
on the cell biology and physiology of skeletal muscle regeneration. This Book
is a collection of classic and cutting edge protocols optimized for mice, but
in most cases adaptable to rat or other mammalian models, that will allow an
investigator to develop and implement a research study on skeletal muscle
regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the
progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by
eccentric contraction; volumetric muscle loss; single myofiber isolation and
culture; satellite cell transplantation; muscle clearing for whole mount
immunostaining; luciferase tracking of muscle stem cells; mitochondrial
and mitophagy flux analysis; in vivo assessment of muscle contractility;
force measurements on single isolated myofibers; and analysis of aerobic
respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly
successful Methods in Molecular Biology series format,
chapters include introductions to each respective topic, lists of the necessary
materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and
practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in
skeletal muscle growth, damage, repair, degeneration, and regenerative therapy
in the mouse model system.
- Vydavateľstvo: Springer New York
- Rok vydania: 2016
- Formát: Hardback
- Rozmer: 260 x 183 mm
- Jazyk: Anglický jazyk
- ISBN: 9781493938087
